School of Analytical Chemistry

10 published lessons · Peptide Chemistry Foundations

Lesson order

1. Lesson 1 · 28–34 min · Beginner–Intermediate

   Introduction to Analytical Chemistry

   Learn how analytical chemists identify, measure, validate, and interpret chemical substances using sampling, calibration, quality control, and complementary analytical techniques.

2. Lesson 2 · 36–44 min · Intermediate

   High-Performance Liquid Chromatography (HPLC)

   Understand how HPLC separates peptide mixtures, how the instrument works, how chromatograms are interpreted, and how method variables affect resolution, retention, and peak shape.

3. Lesson 3 · 38–46 min · Intermediate–Advanced

   Liquid Chromatography–Mass Spectrometry (LC-MS)

   Learn how LC-MS combines chromatographic separation with ionization and mass analysis to confirm peptide identity, characterize impurities, and investigate degradation products.

4. Lesson 4 · 40–48 min · Intermediate–Advanced

   Analytical Method Validation

   Understand how laboratories demonstrate that analytical procedures are fit for purpose through accuracy, precision, specificity, linearity, range, robustness, detection capability, and documented validation.

5. Lesson 5 · 30–36 min · Intermediate

   System Suitability Testing

   Learn how system suitability verifies current analytical readiness through resolution, precision, retention, efficiency, peak symmetry, and documented pass-fail criteria before sample analysis.

6. Lesson 6 · 36–44 min · Intermediate–Advanced

   Measurement Uncertainty

   Learn how laboratories identify, estimate, combine, and report sources of uncertainty so analytical results can be interpreted with scientifically justified confidence.

7. Lesson 7 · 34–42 min · Intermediate

   Chromatographic Peak Integration

   Understand how chromatography software defines peak boundaries, estimates baselines, calculates areas, manages reintegration, and converts detector signals into quantitative results.

8. Lesson 8 · 38–46 min · Intermediate

   Analytical Data Integrity

   Learn how ALCOA+ principles, raw data, metadata, audit trails, access controls, review, backup, and governance preserve trustworthy analytical evidence throughout its lifecycle.

9. Lesson 9 · 40–48 min · Advanced

   Forced Degradation Studies

   Understand how controlled thermal, oxidative, hydrolytic, photolytic, humidity, and mechanical stress studies reveal degradation pathways and support analytical development.

10. Lesson 10 · 40–48 min · Advanced

    Stability-Indicating Methods

    Learn how analytical procedures are developed and validated to distinguish intact peptides from degradation products, impurities, matrix components, and related substances throughout the product lifecycle.