School of Peptide Chemistry
Peptide Synthesis and Manufacturing Foundations
SPPS, protecting groups, coupling chemistry, cleavage/deprotection, and purification for research peptide manufacturing context.
5 lessons
Learning Objectives
- Explain solid-phase peptide synthesis workflows
- Compare protecting-group and coupling strategies
- Describe cleavage, deprotection, and purification considerations
Lesson order
11 of 15 · 34–42 min · Intermediate–Advanced
Solid-Phase Peptide Synthesis (SPPS)Understand the core logic of solid-phase peptide synthesis, including resin attachment, repetitive coupling and deprotection cycles, washing, sequence extension, and common process risks.
12 of 15 · 34–42 min · Advanced
Protecting Groups in Peptide SynthesisLearn why peptide synthesis requires temporary and side-chain protecting groups, how orthogonality works, and how protection strategy affects yield, selectivity, impurity formation, and final deprotection.
13 of 15 · 36–44 min · Advanced
Peptide Coupling ChemistryUnderstand how amino-acid carboxyl groups are activated for peptide-bond formation, how coupling efficiency is assessed, and how racemization, incomplete reaction, and reagent choice influence quality.
14 of 15 · 34–42 min · Advanced
Peptide Cleavage and Global DeprotectionLearn how completed peptides are released from solid supports, how side-chain protecting groups are removed, why scavengers are used, and how cleavage conditions shape the crude impurity profile.
15 of 15 · 38–46 min · Advanced
Peptide Purification by Preparative ChromatographyUnderstand how crude peptide mixtures are separated using preparative chromatography, how fractions are evaluated and pooled, and how purity, recovery, resolution, and scale are balanced.