School of Analytical Chemistry
Analytical Chemistry Foundations
Core analytical methods and data practices used to evaluate research peptide identity, purity, and method performance.
5 lessons
Learning Objectives
- Explain qualitative and quantitative analytical goals
- Interpret HPLC and LC-MS concepts for peptide analysis
- Connect method validation and system suitability to laboratory decision-making
Lesson order
1 of 10 · 28–34 min · Beginner–Intermediate
Introduction to Analytical ChemistryLearn how analytical chemists identify, measure, validate, and interpret chemical substances using sampling, calibration, quality control, and complementary analytical techniques.
2 of 10 · 36–44 min · Intermediate
High-Performance Liquid Chromatography (HPLC)Understand how HPLC separates peptide mixtures, how the instrument works, how chromatograms are interpreted, and how method variables affect resolution, retention, and peak shape.
3 of 10 · 38–46 min · Intermediate–Advanced
Liquid Chromatography–Mass Spectrometry (LC-MS)Learn how LC-MS combines chromatographic separation with ionization and mass analysis to confirm peptide identity, characterize impurities, and investigate degradation products.
4 of 10 · 40–48 min · Intermediate–Advanced
Analytical Method ValidationUnderstand how laboratories demonstrate that analytical procedures are fit for purpose through accuracy, precision, specificity, linearity, range, robustness, detection capability, and documented validation.
5 of 10 · 30–36 min · Intermediate
System Suitability TestingLearn how system suitability verifies current analytical readiness through resolution, precision, retention, efficiency, peak symmetry, and documented pass-fail criteria before sample analysis.