Knowledge Connections

Select an object to view prerequisites, next steps, and related resources.

lessonTSMS-ANL-001

Introduction to Analytical Chemistry

lessonTSMS-ANL-002

High-Performance Liquid Chromatography (HPLC)

lessonTSMS-ANL-003

Liquid Chromatography–Mass Spectrometry (LC-MS)

lessonTSMS-ANL-004

Analytical Method Validation

lessonTSMS-ANL-005

System Suitability Testing

lessonTSMS-ANL-006

Measurement Uncertainty

lessonTSMS-ANL-007

Chromatographic Peak Integration

lessonTSMS-ANL-008

Analytical Data Integrity

lessonTSMS-ANL-009

Forced Degradation Studies

lessonTSMS-ANL-010

Stability-Indicating Methods

assessmentTSMS-ASMT-ANL-002

HPLC Knowledge Check

assessmentTSMS-ASMT-PC-001

What Is a Peptide? Knowledge Check

assessmentTSMS-ASMT-QMS-081

Quality Management Systems Knowledge Check

calculatorTSMS-CAL-001

Peptide Molecular Weight Calculator

calculatorTSMS-CAL-002

Dilution Calculator

calculatorTSMS-CAL-003

Percent Recovery Calculator

quality capaTSMS-CAPA-DEMO-001

Demonstration Corrective Action

glossary termTSMS-CARD-0001

Accuracy

glossary termTSMS-CARD-0002

Analyte

glossary termTSMS-CARD-0003

Amino acid

glossary termTSMS-CARD-0004

Assay

glossary termTSMS-CARD-0005

Audit trail

glossary termTSMS-CARD-0006

Baseline

glossary termTSMS-CARD-0007

Calibration

glossary termTSMS-CARD-0008

Chromatogram

glossary termTSMS-CARD-0009

Chromatography

glossary termTSMS-CARD-0010

Deamidation

glossary termTSMS-CARD-0011

Deconvolution

glossary termTSMS-CARD-0012

Disulfide bond

glossary termTSMS-CARD-0013

Electrospray ionization

glossary termTSMS-CARD-0014

Expanded uncertainty

glossary termTSMS-CARD-0015

Forced degradation

glossary termTSMS-CARD-0016

Gradient elution

glossary termTSMS-CARD-0017

Hydrolysis

glossary termTSMS-CARD-0018

HPLC

glossary termTSMS-CARD-0019

Identity

glossary termTSMS-CARD-0020

Impurity

glossary termTSMS-CARD-0021

Ionization

glossary termTSMS-CARD-0022

Isocratic elution

glossary termTSMS-CARD-0023

Isomer

glossary termTSMS-CARD-0024

LC-MS

glossary termTSMS-CARD-0025

Limit of detection

glossary termTSMS-CARD-0026

Limit of quantitation

glossary termTSMS-CARD-0027

Mass-to-charge ratio

glossary termTSMS-CARD-0028

Measurement uncertainty

glossary termTSMS-CARD-0029

Metadata

glossary termTSMS-CARD-0030

Mobile phase

glossary termTSMS-CARD-0031

Monoisotopic mass

glossary termTSMS-CARD-0032

N-terminus

glossary termTSMS-CARD-0033

Oxidation

glossary termTSMS-CARD-0034

Peptide bond

glossary termTSMS-CARD-0035

Precision

glossary termTSMS-CARD-0036

Primary structure

glossary termTSMS-CARD-0037

Reference standard

glossary termTSMS-CARD-0038

Resolution

glossary termTSMS-CARD-0039

Stationary phase

glossary termTSMS-CARD-0040

System suitability

glossary termTSMS-CARD-0041

Amphipathic

glossary termTSMS-CARD-0042

Average mass

glossary termTSMS-CARD-0043

C-terminus

glossary termTSMS-CARD-0044

Counterion

glossary termTSMS-CARD-0045

Dissolution rate

glossary termTSMS-CARD-0046

Equilibrium solubility

glossary termTSMS-CARD-0047

Hydrophobic effect

glossary termTSMS-CARD-0048

Hydrophobic moment

glossary termTSMS-CARD-0049

Isoelectric point

glossary termTSMS-CARD-0050

Net charge

glossary termTSMS-CARD-0051

pKa

glossary termTSMS-CARD-0052

Residue

glossary termTSMS-CARD-0053

Saturation

glossary termTSMS-CARD-0054

Sequence isomer

glossary termTSMS-CARD-0055

Surface adsorption

glossary termTSMS-CARD-0056

Zwitterion

glossary termTSMS-CARD-0057

Hydropathy

glossary termTSMS-CARD-0058

Charge state

glossary termTSMS-CARD-0059

Adduct

glossary termTSMS-CARD-0060

Capping

glossary termTSMS-CARD-0061

Cleavage cocktail

glossary termTSMS-CARD-0062

Coupling reagent

glossary termTSMS-CARD-0063

Deletion sequence

glossary termTSMS-CARD-0064

Fmoc

glossary termTSMS-CARD-0065

Global deprotection

glossary termTSMS-CARD-0066

Linker

glossary termTSMS-CARD-0067

Orthogonal protection

glossary termTSMS-CARD-0068

Preparative HPLC

glossary termTSMS-CARD-0069

Racemization

glossary termTSMS-CARD-0070

Resin loading

glossary termTSMS-CARD-0071

Scavenger

glossary termTSMS-CARD-0072

Solid-phase peptide synthesis

glossary termTSMS-CARD-0073

Test cleavage

glossary termTSMS-CARD-0074

Yield

glossary termTSMS-CARD-0075

Area percent

glossary termTSMS-CARD-0076

Capacity factor

glossary termTSMS-CARD-0077

Extracted ion chromatogram

glossary termTSMS-CARD-0078

Ion suppression

glossary termTSMS-CARD-0079

Linearity

glossary termTSMS-CARD-0080

Repeatability

glossary termTSMS-CARD-0081

Reproducibility

glossary termTSMS-CARD-0082

Robustness

glossary termTSMS-CARD-0083

Specificity

glossary termTSMS-CARD-0084

Tailing factor

glossary termTSMS-CARD-0085

Theoretical plates

glossary termTSMS-CARD-0086

Total ion chromatogram

glossary termTSMS-CARD-0087

Approved supplier list

glossary termTSMS-CARD-0088

CAPA

glossary termTSMS-CARD-0089

Change control

glossary termTSMS-CARD-0090

Certified reference material

glossary termTSMS-CARD-0091

Deviation

glossary termTSMS-CARD-0092

Finding

glossary termTSMS-CARD-0093

Incoming inspection

glossary termTSMS-CARD-0094

Primary reference standard

glossary termTSMS-CARD-0095

Quality agreement

glossary termTSMS-CARD-0096

Quality assurance

glossary termTSMS-CARD-0097

Quality control

glossary termTSMS-CARD-0098

Quarantine

glossary termTSMS-CARD-0099

Requalification

glossary termTSMS-CARD-0100

Supplier qualification

glossary termTSMS-CARD-0101

Working standard

glossary termTSMS-CARD-0102

Combined standard uncertainty

glossary termTSMS-CARD-0103

Coverage factor

glossary termTSMS-CARD-0104

Decision rule

glossary termTSMS-CARD-0105

Mass balance

glossary termTSMS-CARD-0106

Peak purity

glossary termTSMS-CARD-0107

Reintegration

glossary termTSMS-CARD-0108

Type A evaluation

glossary termTSMS-CARD-0109

Type B evaluation

coaTSMS-COA-DEMO-001

TSMS-COA-DEMO-001

quality deviationTSMS-DEV-DEMO-001

Demonstration Documentation Deviation

figureTSMS-FIG-ANL-001-01

Analytical workflow

figureTSMS-FIG-ANL-001-02

Accuracy versus precision

figureTSMS-FIG-ANL-002-01

HPLC flow path

figureTSMS-FIG-ANL-002-02

Annotated chromatogram

figureTSMS-FIG-ANL-003-01

LC-MS workflow

figureTSMS-FIG-ANL-003-02

Charge states and deconvolution

figureTSMS-FIG-ANL-004-01

Validation lifecycle

figureTSMS-FIG-ANL-004-02

Validation characteristic map

figureTSMS-FIG-ANL-005-01

System suitability workflow

figureTSMS-FIG-ANL-005-02

Suitability failure decision tree

figureTSMS-FIG-ANL-006-01

Uncertainty source map

figureTSMS-FIG-ANL-006-02

Decision near specification limit

figureTSMS-FIG-ANL-007-01

Peak anatomy and baseline

figureTSMS-FIG-ANL-007-02

Reintegration audit trail

figureTSMS-FIG-ANL-008-01

ALCOA+ framework

figureTSMS-FIG-ANL-008-02

Analytical data lifecycle

figureTSMS-FIG-ANL-009-01

Forced degradation matrix

figureTSMS-FIG-ANL-009-02

Degradation-product identification

figureTSMS-FIG-ANL-010-01

Specific versus non-specific chromatogram

figureTSMS-FIG-ANL-010-02

Stability-method lifecycle

figureTSMS-FIG-PC-001-01

General amino-acid structure

figureTSMS-FIG-PC-001-02

Peptide-bond formation

figureTSMS-FIG-PC-002-01

Amino-acid side-chain classes

figureTSMS-FIG-PC-002-02

L and D stereochemistry

figureTSMS-FIG-PC-003-01

Peptide-bond resonance

figureTSMS-FIG-PC-003-02

Cis versus trans peptide bonds

figureTSMS-FIG-PC-004-01

Sequence direction and numbering

figureTSMS-FIG-PC-004-02

Same composition, different sequence

figureTSMS-FIG-PC-005-01

Primary versus higher-order structure

figureTSMS-FIG-PC-005-02

Conformational ensemble

figureTSMS-FIG-PC-006-01

Average versus monoisotopic mass

figureTSMS-FIG-PC-006-02

Charge states and deconvolution

figureTSMS-FIG-PC-007-01

Net charge versus pH

figureTSMS-FIG-PC-007-02

Solubility risk near pI

figureTSMS-FIG-PC-008-01

Hydrophobic clusters in sequence

figureTSMS-FIG-PC-008-02

Reversed-phase interaction

figureTSMS-FIG-PC-009-01

Dissolution rate versus equilibrium solubility

figureTSMS-FIG-PC-009-02

Aggregation and precipitation continuum

figureTSMS-FIG-PC-010-01

Amphipathic helix

figureTSMS-FIG-PC-010-02

Helical-wheel projection

figureTSMS-FIG-PC-011-01

SPPS cycle

figureTSMS-FIG-PC-011-02

Deletion-sequence formation

figureTSMS-FIG-PC-012-01

Fmoc versus Boc logic

figureTSMS-FIG-PC-012-02

Orthogonal protection matrix

figureTSMS-FIG-PC-013-01

Carboxyl activation and amide formation

figureTSMS-FIG-PC-013-02

Racemization risk

figureTSMS-FIG-PC-014-01

Cleavage cocktail functions

figureTSMS-FIG-PC-014-02

Crude peptide impurity profile

figureTSMS-FIG-PC-015-01

Preparative purification workflow

figureTSMS-FIG-PC-015-02

Purity versus recovery tradeoff

figureTSMS-FIG-QMS-081-01

QMS process map

figureTSMS-FIG-QMS-081-02

QA versus QC

figureTSMS-FIG-QMS-082-01

Supplier qualification lifecycle

figureTSMS-FIG-QMS-082-02

Supplier risk matrix

figureTSMS-FIG-QMS-083-01

Vendor audit lifecycle

figureTSMS-FIG-QMS-083-02

Finding classification matrix

figureTSMS-FIG-QMS-084-01

Material qualification workflow

figureTSMS-FIG-QMS-084-02

Reduced-testing decision tree

figureTSMS-FIG-QMS-085-01

Reference-standard hierarchy

figureTSMS-FIG-QMS-085-02

Traceability chain

study guideTSMS-GDE-TSMS-ANL-001

Introduction to Analytical Chemistry Study Guide

study guideTSMS-GDE-TSMS-ANL-002

High-Performance Liquid Chromatography (HPLC) Study Guide

study guideTSMS-GDE-TSMS-ANL-003

Liquid Chromatography–Mass Spectrometry (LC-MS) Study Guide

study guideTSMS-GDE-TSMS-ANL-004

Analytical Method Validation Study Guide

study guideTSMS-GDE-TSMS-ANL-005

System Suitability Testing Study Guide

study guideTSMS-GDE-TSMS-ANL-006

Measurement Uncertainty Study Guide

study guideTSMS-GDE-TSMS-ANL-007

Chromatographic Peak Integration Study Guide

study guideTSMS-GDE-TSMS-ANL-008

Analytical Data Integrity Study Guide

study guideTSMS-GDE-TSMS-ANL-009

Forced Degradation Studies Study Guide

study guideTSMS-GDE-TSMS-ANL-010

Stability-Indicating Methods Study Guide

study guideTSMS-GDE-TSMS-PC-001

What Is a Peptide? Study Guide

study guideTSMS-GDE-TSMS-PC-002

Amino Acids: Structure, Classification, and Chemical Behavior Study Guide

study guideTSMS-GDE-TSMS-PC-003

Peptide Bonds: Formation, Geometry, and Chemical Stability Study Guide

study guideTSMS-GDE-TSMS-PC-004

Primary Structure: How Sequence Defines Peptide Identity Study Guide

study guideTSMS-GDE-TSMS-PC-005

Higher-Order Peptide Structure Study Guide

study guideTSMS-GDE-TSMS-PC-006

Peptide Molecular Weight and Mass Calculation Study Guide

study guideTSMS-GDE-TSMS-PC-007

Peptide Charge, pKa, and Isoelectric Point Study Guide

study guideTSMS-GDE-TSMS-PC-008

Peptide Hydrophobicity and Molecular Interactions Study Guide

study guideTSMS-GDE-TSMS-PC-009

Peptide Solubility: Chemical Drivers and Analytical Considerations Study Guide

study guideTSMS-GDE-TSMS-PC-010

Amphipathic Peptides and Interfacial Behavior Study Guide

study guideTSMS-GDE-TSMS-PC-011

Solid-Phase Peptide Synthesis (SPPS) Study Guide

study guideTSMS-GDE-TSMS-PC-012

Protecting Groups in Peptide Synthesis Study Guide

study guideTSMS-GDE-TSMS-PC-013

Peptide Coupling Chemistry Study Guide

study guideTSMS-GDE-TSMS-PC-014

Peptide Cleavage and Global Deprotection Study Guide

study guideTSMS-GDE-TSMS-PC-015

Peptide Purification by Preparative Chromatography Study Guide

study guideTSMS-GDE-TSMS-QMS-081

Introduction to Quality Management Systems Study Guide

study guideTSMS-GDE-TSMS-QMS-082

Supplier Qualification Study Guide

study guideTSMS-GDE-TSMS-QMS-083

Vendor Audits Study Guide

study guideTSMS-GDE-TSMS-QMS-084

Raw Material Qualification Study Guide

study guideTSMS-GDE-TSMS-QMS-085

Reference Standards Study Guide

lotTSMS-LOT-DEMO-001

Lot DEMO-ONLY

learning pathTSMS-PATH-001

Peptide Chemistry Foundations

learning pathTSMS-PATH-002

Analytical Chemistry Core

learning pathTSMS-PATH-003

Quality Management Foundations

lessonTSMS-PC-001

What Is a Peptide?

lessonTSMS-PC-002

Amino Acids: Structure, Classification, and Chemical Behavior

lessonTSMS-PC-003

Peptide Bonds: Formation, Geometry, and Chemical Stability

lessonTSMS-PC-004

Primary Structure: How Sequence Defines Peptide Identity

lessonTSMS-PC-005

Higher-Order Peptide Structure

lessonTSMS-PC-006

Peptide Molecular Weight and Mass Calculation

lessonTSMS-PC-007

Peptide Charge, pKa, and Isoelectric Point

lessonTSMS-PC-008

Peptide Hydrophobicity and Molecular Interactions

lessonTSMS-PC-009

Peptide Solubility: Chemical Drivers and Analytical Considerations

lessonTSMS-PC-010

Amphipathic Peptides and Interfacial Behavior

lessonTSMS-PC-011

Solid-Phase Peptide Synthesis (SPPS)

lessonTSMS-PC-012

Protecting Groups in Peptide Synthesis

lessonTSMS-PC-013

Peptide Coupling Chemistry

lessonTSMS-PC-014

Peptide Cleavage and Global Deprotection

lessonTSMS-PC-015

Peptide Purification by Preparative Chromatography

packaging recordTSMS-PKG-001

Research vial with approved closure and label

productTSMS-PRD-001

5-Amino-1MQ

lessonTSMS-QMS-081

Introduction to Quality Management Systems

lessonTSMS-QMS-082

Supplier Qualification

lessonTSMS-QMS-083

Vendor Audits

lessonTSMS-QMS-084

Raw Material Qualification

lessonTSMS-QMS-085

Reference Standards

referenceTSMS-REF-01D9616205

Eurachem. The Fitness for Purpose of Analytical Methods.

referenceTSMS-REF-0845BF506C

Behrendt R, White P, Offer J. Advances in Fmoc SPPS.

referenceTSMS-REF-0B28401B28

Mant CT, Hodges RS. High-performance liquid chromatography of peptides and proteins.

referenceTSMS-REF-17123D1A35

Kyte J, Doolittle RF. Hydropathy analysis.

referenceTSMS-REF-1A22465DD3

King DS, Fields CG, Fields GB. Cleavage and deprotection in Fmoc SPPS.

referenceTSMS-REF-20FA79A34F

ISO/IEC 17025. General Requirements for Testing and Calibration Laboratories.

referenceTSMS-REF-28ADE800D5

Pauling L, Corey RB. Configurations of polypeptide chains.

referenceTSMS-REF-2D3C48654E

Pace CN, Grimsley GR, Scholtz JM. Protein ionizable groups.

referenceTSMS-REF-2E42DD0BC7

ISO Guide 30. Reference Materials — Selected Terms and Definitions.

referenceTSMS-REF-2F69685321

ICH Q9. Quality Risk Management.

referenceTSMS-REF-31C2BE332E

MHRA. GxP Data Integrity Guidance and Definitions.

referenceTSMS-REF-380401FCE7

ICH Q2(R2).

referenceTSMS-REF-3A05249D82

Berg JM, Tymoczko JL, Gatto GJ, Stryer L. Biochemistry.

referenceTSMS-REF-4494EACB21

Wang W. Protein aggregation and stability.

referenceTSMS-REF-458F8C5D1C

ICH Q1A(R2). Stability Testing of New Drug Substances and Products.

referenceTSMS-REF-467AE93373

USP <621>.

referenceTSMS-REF-46D6F642C0

Dong MW. Modern HPLC for Practicing Scientists.

referenceTSMS-REF-4A64A37362

Dyson HJ, Wright PE. Intrinsically unstructured proteins and their functions.

referenceTSMS-REF-4E8FE3BEE0

USP General Chapter <1225>, Validation of Compendial Procedures.

referenceTSMS-REF-5DDDC3899D

Harris DC. Quantitative Chemical Analysis.

referenceTSMS-REF-6005E0822A

Biemann K. Mass spectrometric peptide sequencing.

referenceTSMS-REF-61B2C2D694

PIC/S. Good Practices for Data Management and Integrity.

referenceTSMS-REF-6537454D5E

Carpino LA, Han GY. Fmoc amino-protecting group chemistry.

referenceTSMS-REF-65624034FA

USP General Chapter <11>, USP Reference Standards.

referenceTSMS-REF-6B011E24D2

de Hoffmann E, Stroobant V. Mass Spectrometry: Principles and Applications.

referenceTSMS-REF-76F9D00C07

Fields GB, Noble RL. Solid phase peptide synthesis utilizing Fmoc chemistry.

referenceTSMS-REF-77C05A912C

ICH Q14.

referenceTSMS-REF-7AB6EA8803

Aguilar MI. HPLC of Peptides and Proteins.

referenceTSMS-REF-7B0F2B438A

Behrendt R, White P, Offer J. Advances in Fmoc solid-phase peptide synthesis.

referenceTSMS-REF-7BEB4CF8A3

ISO/IEC 17025.

referenceTSMS-REF-8121B465AB

Florence AT, Attwood D. Physicochemical Principles of Pharmacy.

referenceTSMS-REF-8672ABB17C

FDA. Data Integrity and Compliance With Drug CGMP.

referenceTSMS-REF-874497A632

ICH Q1B.

referenceTSMS-REF-8C27F1C57A

Eurachem. Quantifying Uncertainty in Analytical Measurement.

referenceTSMS-REF-952401D32F

ISO/IEC 17025. General Requirements for the Competence of Testing and Calibration Laboratories.

referenceTSMS-REF-959D52D7D9

ICH Q2(R2). Validation of Analytical Procedures.

referenceTSMS-REF-973A2DFF1E

USP General Chapter <621>, Chromatography.

referenceTSMS-REF-97697EDFBD

Eisenberg D, Weiss RM, Terwilliger TC. Hydrophobic moment and amphiphilicity.

referenceTSMS-REF-987B8EA9A6

Nelson DL, Cox MM. Lehninger Principles of Biochemistry.

referenceTSMS-REF-9C6E2CE710

ISO 19011. Guidelines for Auditing Management Systems.

referenceTSMS-REF-9CB4745A9E

Dass C. Fundamentals of Contemporary Mass Spectrometry.

referenceTSMS-REF-9DD540252C

ICH Q14. Analytical Procedure Development.

referenceTSMS-REF-A1E238956A

ICH Q7. Good Manufacturing Practice Guide for Active Pharmaceutical Ingredients.

referenceTSMS-REF-A201D546DF

ICH Q1A(R2).

referenceTSMS-REF-A2BE2B785F

Creighton TE. Proteins: Structures and Molecular Properties.

referenceTSMS-REF-A635B9F952

Isidro-Llobet A, Álvarez M, Albericio F. Protecting-group chemistry.

referenceTSMS-REF-A802259A36

JCGM 100. Guide to the Expression of Uncertainty in Measurement.

referenceTSMS-REF-AAE90A3097

ISO 9001. Quality Management Systems — Requirements.

referenceTSMS-REF-AE949CCD98

Greene TW, Wuts PGM. Protective Groups in Organic Synthesis.

referenceTSMS-REF-B215687605

Valeur E, Bradley M. Amide bond formation.

referenceTSMS-REF-B6FEB8200D

Snyder LR, Kirkland JJ, Dolan JW. Introduction to Modern Liquid Chromatography.

referenceTSMS-REF-B93B48FD29

ICH Q10. Pharmaceutical Quality System.

referenceTSMS-REF-BFEEA3BFEF

Tanford C. The Hydrophobic Effect.

referenceTSMS-REF-C9370A2B9D

Greenfield NJ. Circular dichroism analysis for protein and peptide structure.

referenceTSMS-REF-CB69CCD1A8

Wüthrich K. NMR of Proteins and Nucleic Acids.

referenceTSMS-REF-CF73F67F7A

Gross JH. Mass Spectrometry: A Textbook.

referenceTSMS-REF-D205B3BB62

Biemann K. Sequencing of peptides by tandem mass spectrometry.

referenceTSMS-REF-D3968091A3

Merrifield RB. Solid phase peptide synthesis. J Am Chem Soc. 1963.

referenceTSMS-REF-E51E20BEC5

White SH, Wimley WC. Membrane partitioning of peptides.

referenceTSMS-REF-E89075F3B9

ISO Guide 35. Reference Materials — Guidance for Characterization and Assessment of Homogeneity and Stability.

referenceTSMS-REF-F10420534F

El-Faham A, Albericio F. Peptide coupling reagents.

referenceTSMS-REF-F32F546DCF

Chan WC, White PD. Fmoc Solid Phase Peptide Synthesis.

referenceTSMS-REF-F6E5311B53

Isidro-Llobet A, Álvarez M, Albericio F. Amino acid protecting groups.

referenceTSMS-REF-F704F1BB31

Skoog DA, Holler FJ, Crouch SR. Principles of Instrumental Analysis.

simulationTSMS-SIM-001

Chromatography Separation Explorer

simulationTSMS-SIM-002

Calibration Curve Explorer

simulationTSMS-SIM-003

Pipetting Accuracy and Precision Explorer

specificationTSMS-SPEC-001