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HPLC Knowledge Check

Review lesson: High-Performance Liquid Chromatography (HPLC)

1. What produces chromatographic separation in HPLC?Differential interaction with stationary and mobile phasesPressure aloneDetector wavelength aloneSample color

2. Why is gradient elution common for peptide mixtures?Peptides can span a wide range of hydrophobicityIt eliminates the need for a columnIt makes every peak identicalIt proves molecular identity

3. Does one large HPLC peak alone prove absolute purity?Yes, alwaysNo, co-elution and detector limitations remain possibleOnly when pressure is highOnly when retention time is short

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